Attenuating the potential hepatotoxicity of Psoraleae Fructus by pre-processing: the alcohol soaking and water rinsing method
10.16438/j.0513-4870.2019-0645
- VernacularTitle:补骨脂潜在肝毒性的修制减毒方法研究: 酒浸水漂法
- Author:
Di SONG
1
,
2
;
Shuai-shuai CHEN
1
,
2
;
Peng-yan LI
3
;
Le ZHANG
1
,
2
;
Zhao-fang BAI
3
;
Xiao-he XIAO
3
;
Xu-hua QIN
1
;
Jia-bo WANG
3
Author Information
1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. China Military Institute of Chinese Medicine, the Fifth Medical Centre of Chinese PLA General Hospital, Beijing 100039, China
3. China Military Institute of Chinese Medicine, the Fifth Medical Centre of Chinese PLA General Hospital, Beijing 100039, China
- Publication Type:Research Article
- Keywords:
Psoraleae Fructus;
hepatotoxicity;
pre-processing;
attenuation;
alcohol soaking and water rinsing;
uniform design;
liver organoid
- From:
Acta Pharmaceutica Sinica
2020;55(2):276-282
- CountryChina
- Language:Chinese
-
Abstract:
Recently, the hepatotoxicity issue regarding to Psoraleae Fructus (PF) has attracted remarkable concerns, which highlights the urgent need to explore the toxicity attenuation method for PF. In this study, we proposed an alcohol soaking and water rinsing method for pre-processing PF based on the record in the classics - "Lei Gong Pao Zhi Lun", aiming to attenuate the potential hepatotoxicity of PF. The optimal pre-processing methods and parameters were investigated by U*12(108) uniform design coupled with 3D-cultured human-derived liver organoids model and high-content imaging. The results showed that there were significant variations among the hepatotoxicity intensities of different pre-processed PF products. Four factors, including the concentration of alcohol, the ratio of material and alcohol in alcohol soaking, the time of alcohol soaking and the times of water rinsing, were found as independent significant factors (all P<0.01). The optimal pre-process parameters were further predicted and verified as follows: the alcohol concentration is 80%, the times of alcohol soaking is 3, the ratio of alcohol and material of alcohol soaking is 3, the time for alcohol soaking is 30 h, the ratio of water and material of water rinsing is 2, the times of water rinsing is 3, the time water rinsing is 12 h and the time of steaming is 5 h. This research demonstrated that the alcohol soaking and water rinsing method can effectively reduce the potential hepatotoxicity of PF. This method provides a reference for reducing the risk of PF liver injury from the perspective of Chinese medicinal materials pre-processing.
