Effects of Guilingji on serum metabolites in D-galactose-induced aging in rats
10.16438/j.0513-4870.2019-0622
- VernacularTitle:龟龄集对D-半乳糖致衰老大鼠血清代谢组学的影响
- Author:
Xiao-ling YANG
1
;
Si-jun ZHAO
1
,
2
;
Jun-sheng TIAN
1
;
Bin ZHANG
3
;
Pei-yi WANG
3
;
Xiao-xia GAO
1
;
Xue-mei QIN
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
2. Shanxi Food and Drug Inspection Institute, Taiyuan 030006, China
3. Shanxi Guang Yuyuan Traditional Chinese Medicine Co., Ltd., Taigu 030800, China
- Publication Type:Research Article
- Keywords:
Guilingji;
elaying senescence;
open field test;
LC-MS;
metabolomics
- From:
Acta Pharmaceutica Sinica
2020;55(2):315-322
- CountryChina
- Language:Chinese
-
Abstract:
This work investigates the effects of Guilingji (GLJ) on D-galactose-induced aging and changes in serum metabolites by UHPLC-Q exactive orbitrap-MS in rats. The rat model of aging by subcutaneous injection of D-galactose (300 mg·kg-1) was used to analyze the effect of different concentrations of GLJ (37.5, 75, 150 mg·kg-1) on an open field test in aging rats. Rat serum was collected after 8 weeks and subjected to LC-MS to analyze the anti-aging effect of GLJ. Animal experimentation was approved according to the Committee on the Ethics of Animal Experiments of Shanxi University (SXULL2014032). GLJ significantly improved the autonomous activity of rats. Compared with the control group, 23 metabolites in the treated group changed significantly, involving three main pathways. The group that was given GLJ had altered regulation of 4 serum metabolites in two pathways. Our results indicate that GLJ can delay aging behavior in rats; the mechanism of this anti-ageing effect remains to be determined.
