Triterpenoids from Galbanumofuygur medicine andtheir anticholinesterase activities
10.16438/j.0513-4870.2019-0484
- VernacularTitle:维药格蓬脂中三萜类化学成分研究及其抗胆碱酯酶活性评价
- Author:
Shu-yun WANG
1
,
2
,
3
;
Fu-zhou SUN
4
;
Yi-fan SUN
5
;
Jian HUANG
3
,
6
;
Jin-hui WANG
3
,
6
;
Bao-feng YANG
6
Author Information
1. School of Pharmacy, Henan University, Kaifeng 475004, China
2. Department of Medicinal Chemistry and Natural Medicine Chemistry (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin 150081, China
3. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
4. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China
5. Shenzhen Honghui Biopharmaceutical Co., Ltd., Shenzhen 518118, China
6. Department of Medicinal Chemistry and Natural Medicine Chemistry (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China), Harbin Medical University, Harbin 150081, China
- Publication Type:Research Article
- Keywords:
Galbanum;
triterpenes;
cholinesterase inhibition activities;
molecular docking
- From:
Acta Pharmaceutica Sinica
2020;55(2):283-288
- CountryChina
- Language:Chinese
-
Abstract:
Eight triterpenes were isolated from the methanol extract of Galbanum by various chromatographic methods including silica gel, ODS opening column, recrystallization and semi-preparative HPLC. Their structures were determined by spectroscopic methods and physicochemical properties as 3β,19α,21α-trihydroxyl-12-en-28-oic acid (1), sumaresinolic acid (2), 3β,19α-dihydroxyl-12-en-28-oic acid (3), oleanolic acid (4), 3β,6β,19α-trihydroxyl-12-en-28-oic acid (5), 19α-hydroxy oleanonic acid (6), 6α-hydroxy oleanonic acid (7), and (11R,12R)-3α,6α-dihydroxy-epoxyolean-28α,13α-olide (8). Among them, compound 1 is a new compound, while compounds 2-8 were newly isolated from the Apiaceae family. The ability of compounds 1-8 to inhibit cholinesterase was determined with an improved Ellman method. Compound 1 showed strong inhibitory activity against butyrylcholinesterase. The molecular docking results indicated that Trp82, His438, Phe329 and Ala328 played an important role in the binding of compound 1 to butyrylcholinesterase.
