Effects of arsenic trioxide on migration, invasion and apoptosis of hepatocellular carcinoma HepG2 cells.
10.7507/1001-5515.201907044
- Author:
Jia HE
1
;
Bowen XU
1
;
Wenbo GAO
1
;
Guanyue SU
1
;
Hongchi YU
2
;
Yang SHEN
1
;
Xiaoheng LIU
1
Author Information
1. Institute of Biomedical Engineering, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, P.R.China.
2. National Engineering Research Center for Biomaterials, Chengdu 610065, P.R China.
- Publication Type:Journal Article
- Keywords:
HepG2 cells;
apoptosis;
arsenic trioxide;
invasion;
migration
- From:
Journal of Biomedical Engineering
2020;37(1):105-111
- CountryChina
- Language:Chinese
-
Abstract:
The article aims to explore the optimal concentration of arsenic trioxide (As O ) on HepG2 of liver cancer cells, and the effect of As O on the migration, invasion and apoptosis of HepG2 cells. In this study, the activity of HepG2 cells treated with 0, 1, 2, 4, 8, 16, 32 μmol/L As O was tested by CCK-8 method, the semi-inhibitory concentration (IC50) was calculated, and the morphological changes of HepG2 cells were observed after the action of As O at IC50 concentration for 12, 24, 48 h. The effect of As O on cell migration and invasion ability was verified by wound healing experiment and Transwell invasion experiment. Western blot and qRT-PCR were used to detect the effects of As O on the gene and protein expression levels related to cell migration, invasion and apoptosis. The results showed that, compared with the control group, the activity of HepG2 cells decreased with the increase of the concentration of As O treatment, showing a dose-dependent effect, and its IC50 was 7.3 μmol/L. After 24 hours' treatment with 8 μmol/L As O , HepG2 cells underwent significant apoptosis, and its migration and invasion abilities were significantly reduced. In addition, the protein expression levels of RhoA, Cdc42, Rac1 and matrix metalloproteinase-9 (MMP-9) were down-regulated, the protein and mRNA expression levels of anti-apoptotic gene Bcl-2 were significantly down-regulated, and the protein and mRNA expression levels of pro-apoptotic genes Bax and Caspase-3 were significantly up-regulated. The above results indicate that certain concentration of As O can inhibit the migration and invasion of hepatocellular carcinoma cells and promote the apoptosis of hepatocellular carcinoma cells.
