In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model
- Author:
Kee Hang LEE
1
;
Hyun NAM
;
Jeong Seob WON
;
Ji Yoon HWANG
;
Hye Won JANG
;
Sun Ho LEE
;
Kyeung Min JOO
Author Information
- Publication Type:Original Article
- Keywords: Spinal cord injuries; Distribution; Lateral ventricles; Cisterna magna; Fluorescence; Optical imaging
- MeSH: Animals; Cell- and Tissue-Based Therapy; Cisterna Magna; Fluorescence; Lateral Ventricles; Models, Animal; Optical Imaging; Rats; Spinal Cord; Spinal Cord Diseases; Spinal Cord Injuries; Treatment Outcome
- From:Journal of Korean Neurosurgical Society 2018;61(4):434-440
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVE: The purpose of this study was to find an optimal delivery route for clinical trials of intrathecal cell therapy for spinal cord injury in preclinical stage.METHODS: We compared in vivo distribution of Cy5.5 fluorescent dye in the spinal cord region at various time points utilizing in vivo optical imaging techniques, which was injected into the lateral ventricle (LV) or cisterna magna (CM) of rats.RESULTS: Although CM locates nearer to the spinal cord than the LV, significantly higher signal of Cy5.5 was detected in the thoracic and lumbar spinal cord region at all time points tested when Cy5.5 was injected into the LV. In the LV injection Cy5.5 signal in the thoracic and lumbar spinal cord was observed within 12 hours after injection, which was maintained until 72 hours after injection. In contrast, Cy5.5 signal was concentrated at the injection site in the CM injection at all time points.CONCLUSION: These data suggested that the LV might be suitable for preclinical injection route of therapeutics targeting the spinal cord to test their treatment efficacy and biosafety for spinal cord diseases in small animal models.
