Expression of Bradykinin Receptors and Effects of Bradykinin in the Interstitial Cells of Cajal from Mouse Small Intestine.
10.11637/kjpa.2004.17.4.289
- Author:
Young Youp KOH
1
;
Jae Yeoul JUN
;
Seok CHOI
;
Yong LIM
;
Jun Soo KIM
;
Hyun Sik MOON
;
Myung Young KIM
;
Kyung Won CHO
;
Hyang Hoon CHO
;
In Youb CHANG
Author Information
1. Department of Anatomy, College of Medicine, Chosun University, Korea. iyjang@mail.chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Bradykinin;
Interstitial cells of Cajal;
Bradykinin receptor;
Gastrointestinal motility
- MeSH:
Animals;
Bradykinin*;
Gastrointestinal Motility;
Immunohistochemistry;
Interstitial Cells of Cajal*;
Intestine, Small*;
Membranes;
Mice*;
Muscle, Smooth;
Patch-Clamp Techniques;
Receptors, Bradykinin*
- From:Korean Journal of Physical Anthropology
2004;17(4):289-295
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Interstitial Cells of Cajal (ICC) are pacemaker cells that generates slow waves and drive spontaneous mechanical contractions of gastrointestinal smooth muscle. Slow waves are generated the periodic activation of spontaneous inward currents (pacemaker currents). We studied the modulation of pacemaker activities by bradykinin (10-8 M) in cultured ICC with the whole cell patch-clamp technique, and the localization of bradykinin-2 receptor-immunoreactivity using double labelling immunohistochemistry in the murine small intestine. Externally applied bradykinin produced membrane depolarization in current-clamping mode. At a -70 mV of holding potential bradykinin increased tonic inward pacemaker currents. Double labelling with bradykinin-2 receptor and and c-kit was shown that ICC expressed the bradykinin-2 receptor-immunoreactivity. These results suggest that bradykinin modulates electrical activities of ICC via bradykinin-2 receptor, which may regulate gastrointestinal motility.
