Research progress and applications of gene editing technology CRISPR/Cas in zebrafish.

Author: Juan OUYANG ¹ ; Songlei XUE ¹ ; Qiqi ZHOU ¹ ; Hengmi CUI ¹
Author Information

1. Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Publication Type:Journal Article
Keywords: CRISPR/Cas; gene editing; zebrafish
From: Chinese Journal of Biotechnology 2020;36(1):1-12
CountryChina
Language:Chinese
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) are acquired immune system in bacteria and archaea. This system is used in site-directed gene editing. Recently, scientists discovered new CRISPR-associated (Cas) proteins, in which Cas12a-mediated gene editing can significantly reduce the off-target rate. In this article, we review CRISPR/Cas system's discovery of history, composition, classification, and working principle. The latest research progress of the CRISPR/Cas system, and its application in zebrafish are introduced.