Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth
10.5933/JKAPD.2019.46.1.85
- Author:
Jihye YUN
1
;
Yong Ouk YOU
;
Eunsuk AHN
;
Jun LEE
;
So Youn AN
Author Information
1. Department of Pediatric Dentistry, College of Dentistry, Wonkwang University, Korea. 9543sue@hanmail.net
- Publication Type:Original Article
- Keywords:
Cytotoxicity;
Calcium silicate;
Mineral trioxide aggregate;
Deciduous tooth;
Stem cells
- MeSH:
Calcium;
Culture Media;
Flow Cytometry;
Humans;
Phenotype;
Stem Cells;
Tooth, Deciduous
- From:
Journal of Korean Academy of Pediatric Dentistry
2019;46(1):85-92
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-Seal™ (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.
