Roles of Sonic Hedgehog Signaling During Tooth Root and Periodontium Formation
10.5933/JKAPD.2018.45.2.144
- Author:
Jaewon HWANG
1
;
Eui sic CHO
;
Yeonmi YANG
Author Information
1. Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Korea.
- Publication Type:Original Article
- Keywords:
Sonic Hedgehog signaling;
Smoothened;
Periodontium
- MeSH:
Animals;
Dental Cementum;
Dental Pulp Cavity;
Dentin;
Hedgehogs;
Mice;
Molar;
Osteocalcin;
Periodontal Ligament;
Periodontium;
Recombinases;
Root Resorption;
Tooth Root;
Tooth
- From:
Journal of Korean Academy of Pediatric Dentistry
2018;45(2):144-153
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of this study was to understand the roles of Sonic Hedgehog (SHH) signaling during tooth root and periodontium formation. In this study, we generated the dental mesenchyme-specific Smoothened (Smo) activated/inactivated mice with the activity of Cre recombinase under the control of osteocalcin promoter.In the Smo activated mutant molar sections at the postnatal 28 days, we found extremely thin root dentin and widened pulp chamber. Picrosirius red staining showed loosely arranged fibers in the periodontal space and decreased cellular cementum with some root resorption. Immunohistochemical staining showed less localization of matrix proteins such as Bsp, Dmp1, Pstn, and Ank in the cementum, periodontal ligament, and/or cementoblast.In the Smo inactivated mutant mouse, there was not any remarkable differences in the localization of these matrix proteins compared with the wild type. These findings suggest that adequate suppressing regulation of SHH signaling is required in the development of tooth root and periodontium.
