Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent
10.5933/JKAPD.2018.45.1.32
- Author:
Jeongeun LEE
1
;
Howon PARK
;
Juhyun LEE
;
Hyunwoo SEO
;
Siyoung LEE
Author Information
1. Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University, Korea. ljh55@gwnu.ac.kr
- Publication Type:Original Article
- Keywords:
3 Tone disclosing agent;
Dental plaque;
Quantitative real-time polymerase chain reaction;
Streptococcus mutans;
Streptococcus sobrinus;
Lactobacillus spp
- MeSH:
Bacteria;
Child;
Dental Caries;
Dental Plaque;
DNA;
Humans;
Lactobacillus;
Real-Time Polymerase Chain Reaction;
Streptococcus mutans;
Streptococcus sobrinus;
Tooth
- From:
Journal of Korean Academy of Pediatric Dentistry
2018;45(1):32-40
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus spp. quantified using a quantitative real-time polymerase chain reaction (qRT-PCR).15 healthy children of ages 9 – 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species.The levels of S. mutans, S. sobrinus, and Lactobacillus spp. were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque.The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans, S. sobrinus, and Lactobacillus spp.
