Medium- and long-chain triglyceride propofol reduces the activity of acetyl-coenzyme A carboxylase in hepatic lipid metabolism in HepG2 and Huh7 cells
10.4196/kjpp.2020.24.1.19
- Author:
Li yuan WANG
1
;
Jing WU
;
Ya fen GAO
;
Duo mao LIN
;
Jun MA
Author Information
1. Center for Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R China. majun7689@163.com
- Publication Type:Original Article
- Keywords:
Hepatocytes;
Liver;
Metabolism;
Propofol
- MeSH:
Acetyl-CoA Carboxylase;
AMP-Activated Protein Kinases;
Cell Survival;
Fatty Acids, Nonesterified;
Hepatocytes;
Intensive Care Units;
Lipid Metabolism;
Liver;
Metabolism;
Phosphorylation;
Propofol;
Triglycerides
- From:The Korean Journal of Physiology and Pharmacology
2020;24(1):19-26
- CountryRepublic of Korea
- Language:English
-
Abstract:
Medium- and long-chain triglyceride (MCT/LCT) propofol is widely used as an intravenous anesthetic, especially in the intensive care unit. The present study aimed to assess whether MCT/LCT propofol is safe in the hyperlipidemic population for long-term use. Free fatty acids (FFAs) were used to establish high-fat stimulation of HepG2 and Huh7 cells. Subsequently, these cells were treated with propofol at the concentration of 0, 4, or 8 µg/ml for 24 and 48 h. The results indicated that the cell viability was notably decreased when the cells were stimulated with 2 mmol/L FFAs and treated with 12 µg/ml MCT/LCT propofol. Accordingly, we chose 2 mmol/L FFAs along with 4 and 8 µg/ml MCT/LCT propofol for the subsequent experiments. Four and 8 µg/ml MCT/LCT propofol inhibited FFA-induced lipid accumulation in the cells and significantly reversed acetyl coenzyme A carboxylase (ACC) activity. In addition, MCT/LCT propofol not only significantly promoted the phosphorylation of AMPK and ACC, but also reversed the FFA-induced decreased phosphorylation of AMPK and ACC. In conclusion, MCT/LCT propofol reverses the negative effects caused by FFAs in HepG2 and Huh7 cells, indicating that MCT/LCT propofol might positively regulate lipid metabolism.
