¹²³I-Labeled oxLDL Is Widely Distributed Throughout the Whole Body in Mice
10.1007/s13139-017-0497-2
- Author:
Atushi NAKANO
1
;
Hidekazu KAWASHIMA
;
Yoshinori MIYAKE
;
Tsutomu ZENIYA
;
Akihide YAMAMOTO
;
Kazuhiro KOSHINO
;
Takashi TEMMA
;
Tetsuya FUKUDA
;
Yoshiko FUJITA
;
Akemi KAKINO
;
Shigehiko KANAYA
;
Tatsuya SAWAMURA
;
Hidehiro IIDA
Author Information
1. Department of Investigative Radiology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan. iida@ri.ncvc.go.jp
- Publication Type:Original Article
- Keywords:
Oxidized low-density lipoprotein (oxLDL);
Radiolabeled ligand;
Dynamic planar imaging;
Biodistribution
- MeSH:
Abdomen;
Animals;
Atherosclerosis;
CHO Cells;
Chromatography, High Pressure Liquid;
Cricetinae;
Fluorescence;
Head Kidney;
Heart;
Inflammation;
Injections, Intravenous;
Iodine;
Kinetics;
Lipoproteins;
Liver;
Lung;
Methods;
Mice;
Radioactivity;
Spleen;
Stomach;
Thyroid Gland;
Urinary Bladder
- From:Nuclear Medicine and Molecular Imaging
2018;52(2):144-153
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Oxidized low-density lipoprotein (oxLDL) plays a key role in endothelial dysfunction, vascular inflammation, and atherogenesis. The aim of this study was to assess blood clearance and in vivo kinetics of radiolabeled oxLDL in mice.METHODS: We synthesized ¹²³I-oxLDL by the iodine monochloride method, and performed an uptake study in CHO cells transfected with lectin-like oxLDL receptor-1 (LOX-1). In addition, we evaluated the consistency between the ¹²³I-oxLDL autoradiogram and the fluorescence image of DiI-oxLDL after intravenous injection for both spleen and liver. Whole-body dynamic planar images were acquired 10 min post injection of ¹²³I-oxLDL to generate regional time-activity curves (TACs) of the liver, heart, lungs, kidney, head, and abdomen. Regional radioactivity for those excised tissues as well as the bladder, stomach, gut, and thyroid were assessed using a gamma counter, yielding percent injected dose (%ID) and dose uptake ratio (DUR). The presence of ¹²³I-oxLDL in serum was assessed by radio-HPLC.RESULTS: The cellular uptakes of ¹²³I-oxLDL were identical to those of DiI-oxLDL, and autoradiograms and fluorescence images also exhibited consistent distributions. TACs after injection of ¹²³I-oxLDL demonstrated extremely fast kinetics. The radioactivity uptake at 10 min postinjection was highest in the liver (40.8 ± 2.4% ID). Notably, radioactivity uptake was equivalent throughout the rest of the body (39.4 ± 2.7% ID). HPLC analysis revealed no remaining ¹²³I-oxLDL or its metabolites in the blood.CONCLUSION: ¹²³I-OxLDL was widely distributed not only in the liver, but also throughout the whole body, providing insight into the pathophysiological effects of oxLDL.
