Evaluation of Polycaprolactone-Associated Human Nasal Chondrocytes as a Therapeutic Agent for Cartilage Repair
10.1007/s13770-019-00210-1
- Author:
Do Hyun KIM
1
;
Mi Hyun LIM
;
Jung Ho JEUN
;
Sun Hwa PARK
;
WeonSun LEE
;
Sang Hi PARK
;
Mi Yeon KWON
;
Se Hwan HWANG
;
Sung Won KIM
Author Information
1. Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of Korea. kswent@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Chondrocyte;
Collagen;
Human nasal septum;
Tissue engineering;
Cartilage
- MeSH:
Adhesives;
Aggrecans;
Cartilage;
Cell Survival;
Chondrocytes;
Collagen;
Collagen Type I;
Fluorescent Antibody Technique;
Humans;
In Vitro Techniques;
Mycoplasma;
Nasal Septum;
Tissue Engineering
- From:
Tissue Engineering and Regenerative Medicine
2019;16(6):605-614
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: In this study, we manufactured a complex of human nasal septal cartilage (hNC) with polycaprolactone (PCL) for transplantation into cartilaginous skeletal defects and evaluated their characteristics.METHODS: Nasal septum tissue was obtained from five patients aged ≥ 20 years who were undergoing septoplasty. hNCs were isolated and subcultured for three passages in vitro. To formulate the cell–PCL complex, we used type I collagen as an adhesive between chondrocyte and PCL. Immunofluorescence staining, cell viability and growth in the hNC–PCL complex, and mycoplasma contamination were assessed.RESULTS: hNCs in PCL showed viability ≥ 70% and remained at these levels for 9 h of incubation at 4 ℃. Immunostaining of the hNC–PCL complex also showed high expression levels of chondrocyte-specific protein, COL2A1, SOX9, and aggrecan during 24 h of clinically applicable conditions.CONCLUSION: The hNC–PCL complex may be a valuable therapeutic agent for implantation into injured cartilage tissue, and can be used clinically to repair cartilaginous skeletal defects. From a clinical perspective, it is important to set the short duration of the implantation process to achieve effective functional implantation.
