Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects
10.1007/s13770-019-00212-z
- Author:
Wei LIU
1
;
Sheng Ning ZHANG
;
Zong Qiang HU
;
Shi Ming FENG
;
Zhen Hui LI
;
Shu Feng XIAO
;
Hong Shu WANG
;
Li LI
Author Information
1. Department of Hepatobillary Surgery, Ganmei Hospital Affiliated to Kunming Medical University, No. 1228 Beijing road, Panlong district, Kunming, Yunnan 650500, China. ynkmlili62@hotmail.com
- Publication Type:Original Article
- Keywords:
Tissue engineering;
Human acellular arterial matrix;
Common bile duct defect
- MeSH:
Bile;
Bile Ducts;
Blood Vessels;
Blotting, Western;
Cholangiopancreatography, Magnetic Resonance;
Cholecystectomy, Laparoscopic;
Coculture Techniques;
Common Bile Duct;
Duodenum;
Epithelial Cells;
Fluorescent Antibody Technique;
Humans;
Infant, Newborn;
Liver;
Methods;
Microscopy, Electron;
Polymerase Chain Reaction;
Swine;
Tissue Engineering
- From:
Tissue Engineering and Regenerative Medicine
2019;16(6):653-665
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects.METHODS: After the decellularization of human arterial blood vessels, the cells were cultured with GFP⁺ (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin-eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot.RESULTS: After 4 weeks (w) of co-culture of HAAM and GFP? porcine bile duct epithelial cells, GFP⁺ porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed.CONCLUSION: The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.
