Identity of Spirometra theileri from a Leopard (Panthera pardus) and Spotted Hyena (Crocuta crocuta) in Tanzania
10.3347/kjp.2019.57.6.639
- Author:
Keeseon S EOM
1
;
Hansol PARK
;
Dongmin LEE
;
Seongjun CHOE
;
Yeseul KANG
;
Mohammed Mebarek BIA
;
Barakaeli Abdieli NDOSI
;
Tilak Chandra NATH
;
Chatanun EAMUDOMKARN
;
Julius KEYYU
;
Robert FYUMAGWA
;
Simon MDUMA
;
Hyeong Kyu JEON
Author Information
1. Department of Parasitology, Parasitology Research Center and Parasite Resource Bank, Chungbuk National University School of Medicine, Cheongju 28644, Korea. jeonhk@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Spirometra theileri;
cox1;
nad1;
morphology;
Tanzania
- MeSH:
Adult;
Animals;
Cestoda;
Electron Transport Complex IV;
Humans;
Hyaenidae;
Intestines;
Male;
NADH Dehydrogenase;
Panthera;
Spirometra;
Tanzania;
Vagina
- From:The Korean Journal of Parasitology
2019;57(6):639-645
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, a Spirometra species of Tanzania origin obtained from an African leopard (Panthera pardus) and spotted hyena (Crocuta crocuta) was identified based on molecular analysis of cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit I (nad1) as well as by morphological observations of an adult tapeworm. One strobila and several segments of a Spirometra species were obtained from the intestine of an African male leopard (Panthera pardus) and spotted hyena (Crocuta crocuta) in the Maswa Game Reserve of Tanzania. The morphological characteristics of S. theileri observed comprised 3 uterine loops on one side and 4 on the other side of the mid-line, a uterine pore situated posterior to the vagina and alternating irregularly either to the right or left of the latter, and vesicular seminis that were much smaller than other Spirometra species. Sequence differences in the cox1 and nad1 genes between S. theileri (Tanzania origin) and S. erinaceieuropaei were 10.1% (cox1) and 12.0% (nad1), while those of S. decipiens and S. ranarum were 9.6%, 9.8% (cox1) and 13.0%, 12.6% (nad1), respectively. The morphological features of the Tanzania-origin Spirometra specimens coincided with those of S. theileri, and the molecular data was also consistent with that of S. theileri, thereby demonstrating the distribution of S. theileri in Tanzania. This places the leopard (Panthera pardus) and spotted hyena (Crocuta crocuta) as new definitive hosts of this spirometrid tapeworm.
