The biofilm removal effect of MnO₂-diatom microbubbler from the dental prosthetic surfaces: In vitro study
10.4047/jkap.2020.58.1.14
- Author:
Eun Hyuk LEE
1
;
Yongbeom SEO
;
Ho Bum KWON
;
Young Jun YIM
;
Hyunjoon KONG
;
Myung Joo KIM
Author Information
1. Department of Prosthodontics, School of Dentistry, Seoul National University, Seoul, Republic of Korea. silk1@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Dental plaque;
Hydrogen peroxide;
MnO₂-diatom microbubbler;
Mouthwash;
Oral hygiene
- MeSH:
Alloys;
Biofilms;
Chlorhexidine;
Decontamination;
Dental Clinics;
Dental Plaque;
Diatoms;
Gentian Violet;
Hydrogen Peroxide;
In Vitro Techniques;
Manganese;
Membranes;
Microscopy, Electron, Scanning;
Mouthwashes;
Oral Hygiene;
Oxygen;
Porphyromonas gingivalis;
Streptococcus mutans
- From:The Journal of Korean Academy of Prosthodontics
2020;58(1):14-22
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The aim of this study is to evaluate the effectiveness of MnO₂-diatom microbubbler (DM) on the surface of prosthetic materials as a mouthwash by comparing the biofilm removal effect with those previously used as a mouthwash in dental clinic.MATERIALS AND METHODS: DM was fabricated by doping manganese dioxide nanosheets to the diatom cylinder surface. Scanning electron microscopy (SEM) was used to observe the morphology of DM and to analyze the composition of doped MnO₂. Stereomicroscope was used to observe the reaction of DM in 3% hydrogen peroxide. Non-precious metal alloys, zirconia and resin specimens were prepared to evaluate the effect of biofilm removal on the surface of prosthetic materials. And then Streptococcus mutans and Porphyromonas gingivalis biofilms were formed on the specimens. When 3% hydrogen peroxide solution and DM were treated on the biofilms, the decontamination effect was compared with chlorhexidine gluconate and 3% hydrogen peroxide solution by crystal violet staining.RESULTS: Manganese dioxide was found on the surface of the diatom cylinder, and it was found to produce bubble of oxygen gas when added to 3% hydrogen peroxide. For all materials used in the experiments, biofilms of the DM-treated groups got effectively removed compared to the groups used with chlorhexidine gluconate or 3% hydrogen peroxide alone.CONCLUSION: MnO₂-diatom microbubbler can remove bacterial membranes on the surface of prosthetic materials more effectively than conventional mouthwashes.
