Asiatic Acid Induces Apoptosis and Autophagy and Reduces MiR-17 and MiR-21 Expression in Pancreatic Cancer Cell Lines
10.20307/nps.2019.25.4.298
- Author:
Yoon Gyung JO
1
;
Myoungjae KIM
;
Hyeji SHIN
;
Ki Yong LEE
;
Eun Joo LEE
Author Information
1. College of Pharmacy and Wonkwang Oriental Medicines Research Institute, Wonkwang University, Iksan, Republic of Korea. ejlee7@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Asiatic acid;
Pancreatic cancer;
Apoptosis;
Autophagy;
MicroRNA
- MeSH:
AMP-Activated Protein Kinases;
Annexin A5;
Apoptosis;
Autophagy;
Blotting, Western;
Cell Line;
Cell Survival;
DNA Nucleotidylexotransferase;
MicroRNAs;
Oncogenes;
Pancreatic Neoplasms;
Sirolimus
- From:Natural Product Sciences
2019;25(4):298-303
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer.
