Development and Validation of HPLC-PDA Method and Pattern Recognition Analyses Using Eight Marker Compounds for the Quality Control Between the Seeds of Cuscuta chinensis Lam. and Cuscuta japonica Choisy
10.20307/nps.2019.25.4.334
- Author:
Duc Hung NGUYEN
1
;
Bing Tian ZHAO
;
Duc Dat LE
;
Eun Sook MA
;
Byung Sun MIN
;
Mi Hee WOO
Author Information
1. College of Pharmacy, Drug Research and Development Center, Daegu Catholic University, Gyeongsan 38430, Republic of Korea. woomh@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Cuscuta chinensis;
Cuscuta japonica;
Flavonoids;
Caffeoylquinic acid;
HPLC-PDA
- MeSH:
Chlorogenic Acid;
Cuscuta;
Diarrhea;
Flavonoids;
Hypertension;
Kidney;
Liver;
Methods;
Quality Control;
Quercetin;
Semen;
Steam
- From:Natural Product Sciences
2019;25(4):334-340
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.
