Antitumor effect of TW-37, a BH3 mimetic in human oral cancer
10.1186/s42826-019-0028-7
- Author:
Chi Hyun AHN
1
;
Won Woo LEE
;
Yun Chan JUNG
;
Ji Ae SHIN
;
Kyoung Ok HONG
;
Sujung CHOI
;
Neeti SWARUP
;
Jihoon KIM
;
Min Hye AHN
;
Minjung JUNG
;
Sung Dae CHO
;
Bohwan JIN
Author Information
1. Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea. efiwdsc@snu.ac.kr
- Publication Type:Original Article
- Keywords:
TW-37;
Apoptosis;
Bcl-2;
Oral cancer
- MeSH:
Animals;
Apoptosis;
Caspase 3;
Cell Line;
Cell Proliferation;
Heterografts;
Humans;
Kidney;
Liver;
Mice;
Mice, Nude;
Mouth Neoplasms;
Myeloid Cells
- From:Laboratory Animal Research
2019;35(4):194-201
- CountryRepublic of Korea
- Language:English
-
Abstract:
TW-37 is a small molecule B cell lymphoma-2 (Bcl-2) homology 3 mimetic with potential anticancer activities. However, the in vivo anti-cancer effect of TW-37 in human oral cancer has not been properly studied yet. Here, we attempted to confirm antitumor activity of TW37 in human oral cancer. TW-37 significantly inhibited cell proliferation and increased the number of dead cells in MC-3 and HSC-3 human oral cancer cell lines. TW-37 enhanced apoptosis of both cell lines evidenced by annexin V/propidium iodide double staining, sub-G1 population analysis and the detection of cleaved poly (ADP-ribose) polymerase and caspase-3. In addition, TW-37 markedly downregulated the expression of Bcl-2 protein, while not affecting Bcl-xL or myeloid cell leukemia-1. In vivo, TW-37 inhibited tumor growth in a nude mice xenograft model without any significant liver and kidney toxicities. Collectively, these data reveal that TW-37 may be a promising small molecule to inhibit human oral cancer.
