Inhibition of STAT3 signaling induces apoptosis and suppresses growth of lung cancer: good and bad
10.1186/s42826-019-0030-0
- Author:
Ju Hee KANG
1
;
Yeong Su JANG
;
Ha Jung LEE
;
Chang Yong LEE
;
Dong Yun SHIN
;
Seung Hyun OH
Author Information
1. College of Pharmacy, Gachon University, Incheon, Republic of Korea. eyeball@hanmail.net
- Publication Type:Original Article
- Keywords:
STX-0119;
STAT3 inhibitor;
NSCLC;
Xenograft;
Cancer
- MeSH:
Animals;
Apoptosis;
Cell Line;
Cell Proliferation;
Cell Survival;
Cyclin D1;
Drug Therapy;
Heterografts;
Humans;
Leukocytes;
Lung Neoplasms;
Lung;
Mice;
Neoplasm Metastasis;
Oncogene Proteins;
Prognosis;
STAT3 Transcription Factor
- From:Laboratory Animal Research
2019;35(4):221-229
- CountryRepublic of Korea
- Language:English
-
Abstract:
Signal transducer and activator of transcription 3 (STAT3) modulates a variety of genes involved in the regulation of critical functions, including cell proliferation, differentiation, apoptosis, angiogenesis, metastasis, and immunity. For many cancers, elevated levels of STAT3 signaling have been associated with a poor prognosis and the development of chemotherapy resistance. In this study, we investigated the inhibitory effects of a novel small-molecule inhibitor of STAT3, STX-0119, on the cell viability and survival of human lung cancer cells. STX-0119 inhibited activated STAT3 and the expression of STAT3-regulated oncoproteins such as c-Myc, cyclin D1, and survivin in lung cancer cells. STX-0119 also decreased the amount of STAT3 in the nuclear fraction as well as induced apoptosis of these lung cancer cell lines as evidenced by increases in apoptotic cells (Annexin V positive) and poly (ADP-ribose) polymerase (PARP) cleavage. The efficacy of STX-0119 in a mouse xenograft model was confirmed. However, a hematological side effect, which had not been previously reported, was observed. The level of white blood cells was significantly lowered when treated at the dose at which STX-0119 alone showed a significant tumor-suppressive effect. In conclusion, we suggest that STX-0119 may be a potent therapeutic agent against lung cancer. Consideration of the side effect suggests, it is necessary to study whether low-dose STX-0119 is effective for lung treatment with a combination of classic lung cancer therapeutics.
