Age-dependent changes of p53 and p63 immunoreactivities in the mouse hippocampus
10.1186/s42826-019-0022-0
- Author:
Tae Kyeong LEE
1
;
Young Eun PARK
;
Cheol Woo PARK
;
Bora KIM
;
Jae Chul LEE
;
Joon Ha PARK
;
Hyang Ah LEE
;
Moo Ho WON
;
Ji Hyeon AHN
Author Information
1. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea. mhwon@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Aging process;
Granule cells;
Mouse;
Hippocampus;
p53;
p63;
Pyramidal neurons
- MeSH:
Adult;
Aging;
Animals;
Cell Aging;
Fluorescence;
Hippocampus;
Humans;
Immunohistochemistry;
Mice;
Neurons;
Pyramidal Cells
- From:Laboratory Animal Research
2019;35(3):140-147
- CountryRepublic of Korea
- Language:English
-
Abstract:
P53 and its family member p63 play important roles in cellular senescence and organismal aging. In this study, p53 and p63 immunoreactivity were examined in the hippocampus of young, adult and aged mice by using immunohistochemistry. In addition, neuronal distribution and degeneration was examined by NeuN immunohistochemistry and fluoro-Jade B fluorescence staining. Strong p53 immunoreactivity was mainly expressed in pyramidal and granule cells of the hippocampus in young mice. p53 immunoreactivity in the pyramidal and granule cells was significantly reduced in the adult mice. In the aged mice, p53 immunoreactivity in the pyramidal and granule cells was more significantly decreased. p63 immunoreactivity was strong in the pyramidal and granule cells in the young mice. p63 immunoreactivity in these cells was apparently and gradually decreased with age, showing that p63 immunoreactivity in the aged granule cells was hardly shown. However, numbers of pyramidal neurons and granule cells were not significantly decreased in the aged mice with normal aging. Taken together, this study indicates that there are no degenerative neurons in the hippocampus during normal aging, showing that p53 and p63 immunoreactivity in hippocampal neurons was progressively reduced during normal aging, which might be closely related to the normal aging processes.
