Effects of Bamboo Salt with Sodium Fluoride on the Prevention of Dental Caries
10.17135/jdhs.2019.19.4.288
- Author:
Hye Jin LEE
1
;
A Reum PARK
;
Han Na OH
Author Information
1. Department of Dental Hygiene, Howon University, Gunsan 54058, Korea. qorrha12@daum.net
- Publication Type:Original Article
- Keywords:
Bamboo salt;
Dental caries;
Sodium fluoride;
Streptococcus mutan
- MeSH:
Bacteria;
Biofilms;
Dental Caries;
Dental Plaque;
Gene Expression;
Glass;
Hydrogen-Ion Concentration;
Mouth;
Real-Time Polymerase Chain Reaction;
Sodium Fluoride;
Sodium;
Spectrophotometry;
Streptococcus mutans
- From:
Journal of Dental Hygiene Science
2019;19(4):288-293
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Dental caries is one of several prevalent oral diseases caused by dental plaque biofilms. This study evaluated the anti-cariogenic effects of a bamboo salt (BS) and sodium fluoride (NaF) mixture on oral bacteria.METHODS: The effects of several mixtures of NaF and BS on acid production, growth, and adhesion to glass beads of Streptococcus mutans, and their anti-cariogenic properties were investigated. The growth of S. mutans was measured according to optical density at 3, 6, 9, 12, 15, 18, and 24 hours after treatment using spectrophotometry at a wavelength of 600 nm, while pH was measured using a pH meter. Adhesion of S. mutans was measured according to the weight of glass beads from each group before and after incubation. Gene expression was measured using real-time polymerase chain reaction. Acid production and growth patterns of S. mutans were compared using repeated measures analysis of variance, followed by Scheffe's post-hoc test. The Kruskal–Wallis test was used to compare adhesion, followed by the Mann–Whitney test. Gene expression in the experimental and control samples was compared using the Student's t-test.RESULTS: Growth, acid production, and adhesion of S. mutans were inhibited in all experimental groups. Expression of gft and fructosyltransferase in S. mutans was inhibited in all groups. A mixture of NaF and BS significantly reduced growth, acid production, adhesion, and gene expression of S. mutans compared with the other groups.CONCLUSION: Results of the present study demonstrated that a mixture of NaF and BS was useful as a mouth rinse in preventing dental caries.
