Prevalence of FLT3 Internal Tandem Duplication in Adult Acute Myelogenous Leukemia.
10.3343/kjlm.2007.27.4.237
- Author:
Jeong Nyeo LEE
1
;
Hye Ran KIM
;
Jeong Hwan SHIN
;
Young Don JOO
Author Information
1. Department of Laboratory Medicine, Busan Paik Hospital, College of Medicine, Korea. jeong418@medimail.co.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Adult;
Acute myelogenous leukemia;
FLT3/ITD
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Chromosomes, Human, Pair 15;
Chromosomes, Human, Pair 17;
Female;
Humans;
Leukemia, Myeloid, Acute/*genetics;
Male;
Middle Aged;
*Mutation;
Polymerase Chain Reaction;
Prognosis;
Survival Analysis;
Tandem Repeat Sequences/*genetics;
fms-Like Tyrosine Kinase 3/*genetics
- From:The Korean Journal of Laboratory Medicine
2007;27(4):237-243
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: fms-like tyrosine kinase (FLT3), a member of the class III receptor tyrosine kinases, regulates the proliferation and differentiation of hematopoietic stem cells. An internal tandem duplication of the FLT3 gene (FLT3/ITD) has been reported in acute myelogenous leukemia (AML) and may be associated with a poor prognosis. In this study we determined the prevalence and prognostic significance of FLT3/ITD in adult AML patients. METHODS: This study included 52 adult de novo AML. Exon 14 and 15 of the FLT3 gene were amplified by PCR and the PCR products were analyzed by 3730XL DNA analyzer (Applied Biosystems, USA) and GeneMapper Software. RESULTS: FLT3/ITD was found in 15 (28.8%) of the 52 AML patients. The presence of FLT3/ITD was significantly associated with absolute leukocyte counts (P=0.002) and bone marrow blast counts (P=0.036). FLT3/ITD was also more frequent in patients with normal karyotype (7 of 18) than in those with cytogenetic aberrations (3 of 25). Patients with t (15;17) showed a higher prevalence of FLT3/ITD (2 of 7). FLT3/ITD was significantly associated with overall survival (P<0.042). CONCLUSIONS: Our data indicate that FLT3/ITD is a common alteration in adult AML patients. Although based on a study with a limited number of AML patients, FLT3/ITD is a prognostic marker in patients with AML.
