Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions
- Author:
Jung Jin LIM
1
;
Hyung Joon KIM
;
Byung Ho RHIE
;
Man Ryul LEE
;
Myeong Jun CHOI
;
Seok Ho HONG
;
Kye Seong KIM
Author Information
- Publication Type:Original Article
- Keywords: Embryonic stem cells; Induced pluripotent stem cells; Feeder-free; Chemically defined conditions; Extracellular matrices
- MeSH: Animals; Cell Culture Techniques; Embryonic Stem Cells; Extracellular Matrix; Feeder Cells; Human Embryonic Stem Cells; Humans; Induced Pluripotent Stem Cells; Pluripotent Stem Cells; Stem Cells
- From:International Journal of Stem Cells 2019;12(3):484-496
- CountryRepublic of Korea
- Language:English
- Abstract: Previously, the majority of human embryonic stem cells and human induced pluripotent stem cells have been derived on feeder layers and chemically undefined medium. Those media components related to feeder cells, or animal products, often greatly affect the consistency of the cell culture. There are clear advantages of a defined, xeno-free, and feeder-free culture system for human pluripotent stem cells (hPSCs) cultures, since consistency in the formulations prevents lot-to-lot variability. Eliminating all non-human components reduces health risks for downstream applications, and those environments reduce potential immunological reactions from stem cells. Therefore, development of feeder-free hPSCs culture systems has been an important focus of hPSCs research. Recently, researchers have established a variety of culture systems in a defined combination, xeno-free matrix and medium that supports the growth and differentiation of hPSCs. Here we described detailed hPSCs culture methods under feeder-free and chemically defined conditions using vitronetin and TeSR-E8 medium including supplement bioactive lysophospholipid for promoting hPSCs proliferation and maintaining stemness.
