In Vitro Cytotoxic Effect of Glyphosate Mixture Containing Surfactants.
10.3346/jkms.2012.27.7.711
- Author:
Ho Yeon SONG
1
;
Young Hee KIM
;
Su Jin SEOK
;
Hyo Wook GIL
;
Sae Yong HONG
Author Information
1. Department of Immunology, Soonchunhyang University College of Medicine, Cheonan, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Glyphosate;
Surfactants;
Complex Mixtures;
Toxicity;
Herbicides
- MeSH:
Animals;
Cell Line;
Cell Survival/drug effects;
Glycine/*analogs & derivatives/chemistry/toxicity;
Herbicides/chemistry/*toxicity;
Mice;
Polyethylene Glycols/*chemistry;
Surface-Active Agents/*chemistry
- From:Journal of Korean Medical Science
2012;27(7):711-715
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated whether glyphosate influences the cellular toxicity of the surfactants TN-20 and LN-10 on the mouse fibroblast-like cells, alveolar epithelial cells, and a heart cell line. The cytotoxicity of TN-20 and LN-10 (0.4-100 microM), in the presence or absence of glyphosate was determined by assessing membrane integrity. TN-20 toxicity was significantly lower in the presence of 50 microM glyphosate for the fibroblast-like cell (6.25 microM; 3.9% +/- 3.4% vs -4.8% +/- 0.7%), for the alveolar cells (0.78 microM; 5.7% +/- 0.9% vs 0.1% +/- 0.6%), and for the heart cell line (25.0 microM; 7.9% +/- 3.0% vs 19.4% +/- 0.7%) compared to that of TN-20 alone. The cellular toxicity of LN-10 towards the fibroblast-like cells was found to be increased in the presence of 50 microM glyphosate when LN-10 concentrations of 50 microM (31.3% +/- 3.9% vs 19.2% +/- 0.9%) and 100 microM (62.1% +/- 3.4% vs 39.0% +/- 0.7%) were compared to that of LN-10 alone. These results suggest that the mixture toxicity may be a factor in glyphosate-surfactant toxicity in patients with acute glyphosate herbicide intoxication.
