Determination of glycine in human thrombin by HPLC
10.3969/j.issn.1006-0111.201903048
- VernacularTitle:用HPLC法测定人凝血酶制品中甘氨酸的含量
- Author:
Fengxian SHENG
1
;
Youjie ZHANG
1
Author Information
1. Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China.
- Keywords:
human thrombin;
glycine;
alanine;
HPLC
- From:
Journal of Pharmaceutical Practice
2020;38(1):74-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for glycine assay in the human thrombin. Methods The sample was derivatized with 2,4-dinitrofluorobenzene (DNFB). HPLC was performed on an ODS-C18 column with 50% acetonitrile –0.05mol/L sodium acetate buffer (35:65) as the mobile phase. The flow rate was 1.0 ml/min and detection wavelength at 360 nm. Results The linear range of glycine was 0.006-0.030 mg/ml (r=0.999 6). The average recovery was 100.4%, with RSD 0.44% (n=9). Conclusion This method is simple, accurate and specific. It is suitable for glycine assay in human thrombin.
