Content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis spectrophotometry
10.3969/j.issn.1006-0111.201904019
- VernacularTitle:紫外-可见分光光度法测定防暑清热饮中总黄酮和总多糖含量
- Author:
Jie LI
1
;
Yuru YANG
1
;
Qingfen WANG
1
;
Shaozhong ZHENG
1
;
Jinshan CHEN
2
Author Information
1. Department of Pharmacy , 909th Hospital of Joint Service Support Force/Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China.
2. Department of Pharmacy , 909th Hospital of Joint Service Support Force/Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China.cjs18659341758@163.com
- Keywords:
UV-Vis spectrophotometry;
Fangshu Qingre mixture;
total flavones;
total polysaccharides;
rutin;
anhydrous glucose
- From:
Journal of Pharmaceutical Practice
2020;38(1):63-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture by UV-Vis Spectrophotometry. Methods The contents of total flavones and polysaccharides in Fangshu Qingre mixture were determined by UV-Vis spectroscopy with rutin and anhydrous glucose as reference substance, and the wavelength was set at 508 nm and 487 nm. Results The contents were from 0.00 to 59.20 μg/ml for total flavones and from 10.92 to 109.20 μg/ml for total polysaccharides in Fangshu Qingre mixture. The recoveries of total flavones and total polysaccharides were 104.4% and 104.8% respectively. Conclusion The method of using ultraviolet spectroscopy was simple, reproducible, accurate and reliable, which could be preferably used as the method for content determination of total flavones and polysaccharides in Fangshu Qingre mixture.
