Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice
10.3969/j.issn.1006-0111.201812070
- VernacularTitle:夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用
- Author:
Minghua MA
1
;
Xiaohe WANG
2
;
Yuanyuan DAI
2
;
Xiaoyan GU
2
;
Tiejun WU
2
;
Hua NIAN
3
Author Information
1. Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China.
2. Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China.
3. Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China.jackynian@126.com
- Keywords:
Chinese Medicine;
Xiakucao Xiaoliu mixture;
Lewis lung cancer;
CyclinD1;
P16
- From:
Journal of Pharmaceutical Practice
2020;38(1):57-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C57BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups: model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.
