Regulation of CYP1A1 and Inflammatory Cytokine by NCOA7 Isoform 4 in Response to Dioxin Induced Airway Inflammation.
- Author:
Sung Hwan CHO
1
;
Shin Young PARK
;
Eun Jeong LEE
;
Yo Han CHO
;
Hyun Sun PARK
;
Seok Ho HONG
;
Woo Jin KIM
Author Information
- Publication Type:Original Article
- Keywords: Receptors, Aryl Hydrocarbon; Pulmonary Disease, Chronic Obstructive; Dioxins; NCOA7 Protein, Human
- MeSH: Cell Line; Cytochrome P-450 CYP1A1*; Cytochromes; Cytokines; Dioxins; Epithelial Cells; Humans; Inflammation*; Interleukin-6; Interleukin-8; Lung; Pulmonary Disease, Chronic Obstructive; Real-Time Polymerase Chain Reaction; Receptors, Aryl Hydrocarbon; Tetrachlorodibenzodioxin; Transcription Factors; Transfection
- From:Tuberculosis and Respiratory Diseases 2015;78(2):99-105
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor, binds to a wide variety of synthetic and naturally occurring compounds. AhR is involved in the regulation of inflammatory response during acute and chronic respiratory diseases. We investigated whether nuclear receptor coactivator 7 (NCOA7) could regulate transcriptional levels of AhR target genes and inflammatory cytokines in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human bronchial epithelial cells. This study was based on our previous study that NCOA7 was differentially expressed between normal and chronic obstructive pulmonary disease lung tissues. METHODS: BEAS-2B and A549 cells grown under serum-free conditions were treated with or without TCDD (0.15 nM and 6.5 nM) for 24 hours after transfection of pCMV-NCOA7 isoform 4. Expression levels of cytochrome P4501A1 (CYP1A1), IL-6, and IL-8 were measured by quantitative real-time polymerase chain reaction. RESULTS: The transcriptional activities of CYP1A1 and inflammatory cytokines were strongly induced by TCDD treatment in both BEAS-2B and A549 cell lines. The NCOA7 isoform 4 oppositely regulated the transcriptional activities of CYP1A1 and inflammatory cytokines between BEAS-2B and A549 cell lines. CONCLUSION: Our results suggest that NCOA7 could act as a regulator in the TCDD-AhR signaling pathway with dual roles in normal and abnormal physiological conditions.
