Expression of SDF-1alpha and leptin, and their effect on expression of angiogenic factors in mouse ovaries.
10.5653/cerm.2011.38.3.135
- Author:
Min Jung PARK
1
;
Sea Hee PARK
;
Su Kyung LEE
;
Sung Eun MOON
;
Hwa Sook MOON
;
Bo Sun JOO
Author Information
1. Center for Reproductive Medicine, Good Moonhwa Hospital, Busan, Korea. bosunjoo@hanmail.net
- Publication Type:Original Article
- Keywords:
Ovary;
Angiogenesis;
Aging;
Stromal Cell Derived Factor-1 Alpha;
Leptin;
Mouse
- MeSH:
Aged;
Aging;
Angiogenesis Inducing Agents;
Animals;
Chemokine CXCL12;
Embryonic Structures;
Female;
Gonadotropins;
Granulosa Cells;
Humans;
Leptin;
Mice;
Mice, Inbred C57BL;
Nicotinamide Phosphoribosyltransferase;
Nitric Oxide Synthase Type III;
Organ Culture Techniques;
Ovary;
RNA, Messenger;
Stromal Cells;
Vascular Endothelial Growth Factor A
- From:Clinical and Experimental Reproductive Medicine
2011;38(3):135-141
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Ovarian angiogenesis plays an important role in folliculogenesis. However, little is known about the expression of angiogenic factors during follicular development according to female age. Stromal cell derived factor-1alpha (SDF-1alpha) plays a role in granulosa cell survival and embryo quality as an angiogenic chemokine. Leptin is also involved in folliculogenesis and angiogenesis. This study examined expression of SDF-1alpha and leptin, and their effects on the expression of angiogenic factors in the ovary during follicular development according to female age. METHODS: Ovaries were collected from C57BL mice of two age groups (6-9 weeks and 24-26 weeks) at 6, 12, 24, and 48 hours after 5 IU pregnant mare's serum gonadotropin (PMSG) injection. The expression of ovarian SDF-1alpha and leptin mRNA was evaluated by RT-PCR. In the organ culture experiment, the ovaries were cultured in transwell permeable supports with Waymouth's medium treated with various doses of SDF-1alpha (50-200 ng/mL) or leptin (0.01-1 microg/mL) for 7 days. Then, mRNA expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and visfatin were examined in the cultured ovaries. RESULTS: Expression of SDF-1alpha and leptin in the ovary was significantly lower in the aged mouse group compared to the young mouse group (p<0.05). Expression of these two factors increased with follicular development after PMSG administration. SDF-1alpha treatment stimulated visfatin expression in a dose-dependent manner, while leptin treatment significantly increased eNOS expression. CONCLUSION: These results suggest that decrease of ovarian SDF-1alpha and leptin expression may be associated with aging-related reduction of ovarian function. SDF-1alpha and leptin may play a role in follicular development by regulating the expression of angiogenic factors in mouse ovaries.
