Efficiency of laser-assisted intracytoplasmic sperm injection in a human assisted reproductive techniques program.
10.5653/cerm.2011.38.3.148
- Author:
Kyoung Hee CHOI
1
;
Jung Hyun LEE
;
Yun Hee YANG
;
Tae Ki YOON
;
Dong Ryul LEE
;
Woo Sik LEE
Author Information
1. Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine, Seoul, Korea. drleedr@cha.ac.kr
- Publication Type:Original Article ; In Vitro ; Randomized Controlled Trial
- Keywords:
Intracytoplasmic Sperm Injection;
Laser-assisted Intracytoplasmic Sperm Injection;
In Vitro Fertilization-embryo Transfer;
Zona Pellucida Thinning;
Clinical Outcome;
Human
- MeSH:
Blastocyst;
Embryonic Development;
Embryonic Structures;
Female;
Fertilization;
Humans;
Oocytes;
Pregnancy;
Pregnancy Rate;
Reproductive Techniques, Assisted;
Sperm Injections, Intracytoplasmic;
Zona Pellucida
- From:Clinical and Experimental Reproductive Medicine
2011;38(3):148-152
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Laser-assisted intracytoplasmic sperm injection (LA-ICSI), also known as micro-opening or thinning of the zona pellucida (ZP) prior to ICSI, may help to reduce mechanical damage to the oocyte during the procedure. The aim of the present study was to evaluate and analyze the efficacy of our institutional LA-ICSI program, which features laser-assisted ZP thinning prior to ICSI, in comparison with conventional ICSI (C-ICSI), performed on patients with different clinical characteristics. METHODS: Patients undergoing a total of 212 ICSI cycles were randomly divided into an LA-ICSI group (106 cycles) and a conventional ICSI group (106 cycles). To reduce tissue damage, we thinned the ZP by approximately 70%, using a laser, before ICSI. Patients thus treated formed the LA-ICSI group. Comparisons included the morphological quality of transferred embryos, blastocyst development of the remaining embryos, and clinical pregnancy, in terms of ICSI method and patient characteristics. RESULTS: Fertilization, development of remaining embryos, and pregnancy rate were significantly higher in the LA-ICSI group compared with the C-ICSI group. Fertilization, embryonic development, and the pregnancy rate were all improved in younger patients (<38 years of age) and in those who underwent a low number of IVF-ET attempts (<3 trials). In addition, the pregnancy rate was increased in older patients. CONCLUSION: LA-ICSI may be useful in improving the chance of pregnancy in all ICSI patients.
