Effect of STIL on the Gene Expression Profile of Gastric Cancer Cells.
10.3881/j.issn.1000-503X.11150
- Author:
Ju WANG
1
;
Xia Zhong DOU
1
;
Qiang Yong WANG
1
;
Wei Hong JIANG
1
;
Wei Hong YUAN
2
Author Information
1. Department of Gastrointestinal Surgery,Inner Mongolia Hospital,Hohhot 010017,China.
2. Teaching and Research Section of Pathological Anatomy,Inner Mongolia Medical University, Hohhot 010010,China.
- Publication Type:Journal Article
- Keywords:
STIL;
bioinformatics;
gastric carcinoma;
gene expression profiling array
- MeSH:
Computational Biology;
Gene Expression Profiling;
Gene Ontology;
Humans;
Stomach Neoplasms;
genetics;
Transcriptome
- From:
Acta Academiae Medicinae Sinicae
2019;41(6):778-786
- CountryChina
- Language:Chinese
-
Abstract:
To explore the molecular mechanism underlying gastric carcinogenesis and progression by using gene expression profiling array together with bioinformatics. Lentivirus short hairpin RNA targeting STIL(ShSTIL)and scrambled sequence RNA(ShCon)were transduced into the gastric cancer cell line SGC-7901.RNA extraction,complementary DNA synthesis,construction of biotin-labelled amplified RNA probes,and hybridization with gene expression profile were consecutively performed.We collected corresponding data and analyzed differentially expressing genes(DEGs),followed by the analysis of gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment,transcription factor regulating network,and protein-protein interacting networks. Compared with ShCon,a total of 417 and 87 genes were respectively down-regulated and up-regulated,respectively,in the ShSTIL group(<0.05,fold change>1 or <-1).GO and KEGG enrichment analysis indicated that genes regulated by STIL were localized in cytoplasm,extracellular exosome,Golgi apparatus and various biomembranes,and were implicated in the ubiquitin-mediated proteolysis,P53 signaling pathway,and pathways regulating pluripotency of stem cells.Evaluation on genes enriched in KEGG pathways,regulation of transcription factors,and protein-protein interacting network demonstrated that IGF1R,STUB1,SKP2,and FOXO1 were localized at the centre of the network and played a key role in the development and progression of gastric cancer. Through the protein-protein interactions,STIL may activate E3 ubiquitin ligase STUB1 or SKP2,promote the proteolysis of FOXO1-a transcription factor,regulate the expression of IGF1R,and thus promote gastric carcinogenesis and progression.
