Practical stability of whole-genome bisulfite sequencing using plasma cell-free DNA.
- Author:
Huan FANG
1
;
Bixi ZHONG
1
;
Lei WEI
1
;
Xianglin ZHANG
1
;
Wei ZHANG
1
;
Xiaowo WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: DNA methylation; fragmentation pattern; low input library; plasma cell-free DNA; whole genome
- MeSH: Cell-Free Nucleic Acids; DNA Methylation; High-Throughput Nucleotide Sequencing; Humans; Sequence Analysis, DNA; Sulfites; Whole Genome Sequencing
- From: Chinese Journal of Biotechnology 2019;35(12):2284-2294
- CountryChina
- Language:Chinese
- Abstract: With the development of liquid biopsy technology, plasma cell-free DNA (cfDNA) becomes one of the research hotspots. Whole-genome bisulfite sequencing of plasma cell-free DNA has shown great potential medical applications such as cancer detection. However, the practical stability evaluation is still lacking. In this study, plasma cell-free DNA samples from two volunteers at different time were collected and prepared for sequencing in multiple laboratories. The library preparation strategies include pre-bisulfite, post-bisulfite and regular whole-genome sequencing. We established a set of quality control references for plasma cell-free DNA sequencing data and evaluated practical stability of blood collection, DNA extraction, and library preparation and sequencing depth. This work provided a technical practice guide for the application of plasma cfDNA methylation sequencing for clinical applications.
