Effect of Pomalidomide on Activity of Myeloma Cell Line MM1.S and Expression of CRBN.
10.7534/j.issn.1009-2137.2019.06.032
- Author:
Wen-Jing FAN
1
;
Zhi-Qiao FAN
1
;
Yao-Zhu PAN
1
;
Ke YANG
1
;
Jiao-Jiao YIN
1
;
Xiao-Chen ZHAO
1
;
Hao YAO
1
;
Hai BAI
2
Author Information
1. Department of Hematology, Center for Hematologic Diseases of Chinese PLA, Lanzhou Military Command General Hospital, Lanzhou 730050, Gansu Proince, China.
2. Department of Hematology, Center for Hematologic Diseases of Chinese PLA, Lanzhou Military Command General Hospital, Lanzhou 730050, Gansu Proince, China,E-mail: baihai98@tom.com.
- Publication Type:Journal Article
- MeSH:
Adaptor Proteins, Signal Transducing;
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Multiple Myeloma;
Thalidomide;
analogs & derivatives
- From:
Journal of Experimental Hematology
2019;27(6):1907-1911
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effects of different concentration of pomalidomide on human multiple myeloma cell line MM1.S and the expression of CRBN.
METHODS:CCK-8 method was used for detecting inhibition effect of promalidomide on proliferation of MM1.S cells. Apoptosis rate of MM1.S cells was detected by flow cytometry with Annexin V-FITC/PI double staining. Real-time quantitative PCR was used to determine CRBN gene expression level. Western blot was used to detect the effect of pomalidomide on the protein expression of CRBN in MM1.S cells.
RESULTS:Pomalidomide has an inhibitory effect on MM1.S cells with time-and dose-dependent manners. Pomalidomide induced apoptosis in MM1.S cells. When the concentration of pomalidomide was 0, 40 and 80 μmol/L, the expression of CRBN gene after the treatment of MM1.S cells for 72 hours was 1.487±0.340, 0.211±0.054 and 0.055±0.005, by using actin as internal refereme. Pomalidomide significantly reduced CRBN protein expression in MM1.S cells.
CONCLUSION:Pomalidomide can inhibit the proliferation of MM1.S cells and promote its apoptosis. A certain concentration of pomalidomide can reduce the expression of CRBN gene and down-regulate its protein expression in MM1.S cells.
