Changes in Metabolism and Function of Platelets Stored at Different Temperature.
10.7534/j.issn.1009-2137.2019.06.048
- Author:
Jie WANG
1
;
Wei-Xin ZHOU
1
;
Ling JIANG
1
;
Si-Min LIANG
2
;
Dong-Mei YANG
2
;
Li YANG
2
;
Qian YANG
2
;
Yuan-Shuai HUANG
3
Author Information
1. Department of Transfusion, Affiliated Hospital of Southwest Medical University,Luzhou 646000, Sichuan Province, China.
2. Department of Clinical Laboratorial Diagnostics, Southwest Medical University, Luzhou 646000, Sichuan Province, China.
3. Department of Transfusion, Affiliated Hospital of Southwest Medical University,Luzhou 646000, Sichuan Province, China,E-mail: hys@1ive.cn.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
Blood Preservation;
Cryopreservation;
Temperature
- From:
Journal of Experimental Hematology
2019;27(6):2003-2008
- CountryChina
- Language:Chinese
-
Abstract:
AAbstractObjective:To compare and analyze the metabolic and functional changes in platelets stored at 4 ℃ and ones stored at 22 ℃ with agitation so as to provide an experimental basis for the cryopreservation technology of platelets.
METHODS:Samples were collected from platelets stored at 4 ℃ in 2, 4, 6, 11, 15 and 21 days, and from ones stored at 22 ℃ with agitation during the same days, the metabolism indicators and thromboelastogram (TEM) were analysed.
RESULTS:In metabolism, there were no significant changes of pH, GLU，PCO2, PCO2 and MPV levels of platelets stored at 4 ℃ for <6 days (P＞0.05), However, the Plt count decreased, the PDW and LDH level incrseased (P＜0.05). At the same time, only MPV had no changes of platelets stored at 22 ℃ during above-mentioned same days (P＞0.05), while the pH, PCO2, GLU, Plt all decreased, and PO2, LDH, PDW incrseased (P＜0.05). There were significant changes about the pH value, PO2, Plt, MPV, LDH, GLU levels between the two kinds of stored platelets during the same storing period (P＜0.05). The pH value and MPV of platelets stored at 4 ℃ were obviously lower than ones stored at 22 ℃, while GLU, PO2, LDH and Plt levels showed reverse changes (P＜0.05). Meanwhile, the PCO2 of platelets stored at 4 ℃ not could be detected and the Plt count reduced rapidly from d15. In function, the MA level of platelets stored at 4 ℃ was slower than that of platelets stored at 22 ℃, that is, the MA level of platelets stored at 4 ℃ were higher than that of platelets stored at 22 ℃ during the same storeing period (P＜0.05).
CONCLUSION:Platelets stored at 4 ℃ have much slower metabolism than ones stored at 22 ℃, and the aggregation is stronger of platelets stored at 4 ℃ than that of ones at 22 ℃ during the same conservation period.
