Effect of B4GALT1 on Proliferation of Its Co-cultured Human Acute Myeloid Leukemia Cell Line in Bone Marrow Stromal Cells.
10.7534/j.issn.1009-2137.2020.01.047
- Author:
Xing-Chen PANG
1
;
Hong-Jiao LI
2
;
Yi WANG
3
;
Feng GUAN
4
;
Xiang LI
5
Author Information
1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province，China.
2. Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an 710069, Shaanxi Province，China.
3. Department of Hematology, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi Province，China.
4. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province，China,Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an 710069, Shaanxi Province，China,E-mail: guanfeng@nwu.edu.cn.
5. Joint International Research Laboratory of Glycobiology and Medicinal Chemistry, College of Life Science, Northwest University, Xi'an 710069, Shaanxi Province，China,E-mail: xiangli@nwu.edu.cn.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2020;28(1):283-289
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of bone marrow stromal cell glycosyltransferase B4GALT1 expression on hematopoietic cell proliferation and its upstream regulation mechanism.
METHODS:B4GALT1 was overexpressed in human bone marrow stromal cell line HS5, which was then co-cultured with acute myeloid leukemia cell line KG1a. And its effect on hematopoietic cell proliferation was detected by flow cytometry. Dual luciferase reporter assay, real-time PCR and Western blot were used to predict and validate upstream transcription factors that regulate stromal cell B4GALT1 expression.
RESULTS:Overexpression of B4GALT1 in HS5 significantly promoted the proliferation of KG1a in the co-culture system. B4GALT1 expression in stromal cells positively correlated with upstream c-Jun expression, which was verified by JNK/c-Jun inhibitors.
CONCLUSION:The differential expression of glycosyltransferases and their corresponding glycosylation in the hematopoietic microenvironment play an important role.
