Establishment and Application of HPA1-6, 15 Platelet Donor Bank in Beijing Area.
10.7534/j.issn.1009-2137.2020.01.049
- Author:
Xiao-Wei WANG
1
;
Chun-Yan SHAO
1
;
Jing ZHANG
1
;
Ling-Ling RENG
1
;
Hai-Long ZHUO
1
;
Qun LUO
2
Author Information
1. Department of Blood Transfusion, Fifth of PLA General Hospital (Former 307 Hospital of PLA), Beijing 100071,China.
2. Department of Blood Transfusion, Fifth of PLA General Hospital (Former 307 Hospital of PLA), Beijing 100071,China,E-mail: luoq66@aliyun.com.
- Publication Type:Journal Article
- From:
Journal of Experimental Hematology
2020;28(1):296-299
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establise the bank of platelet donors with the human platelet antigen (HPA) 1-6, 15 genes so as to provide the HPA-matched platelets for the patients.
METHODS:The HPA genotyping of platelets donors and patients with platelet antibody positive confirmed by sercening was performed by using the SSP-PCR; the efficacy of transfusing the HPA-matched platelets for 37 cases platelet antibody positive was analyzed.
RESULTS:The most common genotype in platelet donors were HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-15a/15b, followed by HPA-1a/1a-2a/2a-3a/3a-4a/4a-5a/5a-6a/6a-15a/15b; the most common genotype in 53 cases of platelet antibody positive confirened by screening were HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-15a/15b. Among 37 patients with platelet antibody positive confirened by screeming, 28 showed that the transfusion of HPA-matched platelets was effective with statistically significant difference in comparison with random transfusion group. The HPA-3, HPA-15 were the main factors leading to polymorphisms.
CONCLUSION:HPA-3 and HPA-15 are polymorphic, which should be focused on. HPA-matched platelets can improve the efficiency of platelet transfusion, and avoid the waste of blood resources. The genotypes of platelet donors can basically meet the requirements for common genotype transfusion.
