Expression Level and Clinical Value of PTEN Gene in Patients with Acute T Lymphocytic Leukemia.
10.7534/j.issn.1009-2137.2019.06.003
- Author:
Zhi-Yuan PENG
1
;
Chun-Xiu YANG
1
;
Jing-Hui SAN
1
;
Shui-Yu LIU
1
;
Ping YUAN
1
;
Bin SHI
2
Author Information
1. Department of Hematology, The Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China.
2. Department of Clinical Laboratorial Examination, The Affiliated Hospital of Zunyi Medical University/Laboraorial College of Zunyi Medical University, Zunyi 563003, Guizhou Province, China,E-mail：shibin_superman@163.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow;
Humans;
Leukemia-Lymphoma, Adult T-Cell;
PTEN Phosphohydrolase;
Prognosis
- From:
Journal of Experimental Hematology
2019;27(6):1722-1727
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the PTEN gene expression level and its clinical significance in patients with acute T lymphoblastic leukemia.
METHODS:One hundred and twenty-four patients with T-ALL treated in our hospital from January 2014 to May 2018 were selected, and 120 healthy people were selected as control group. The bone marrow of the patients were collected, and mononuclear cells were separated out. PTEN gene expression level was detected by RT-PCR, PTEN protein expression level was detected by Western blot, Kaplan-Meier was used to analyze the survival rate of patients with T-ALL, Cox multivariate regression analyzed was used to analyze the independent influencing factors of patients, and the correlation between PTEN level and clinical characteristics of patients with T-ALL and its prognostic value were analyzed.
RESULTS:The relative level of PTEN gene in patients with T-ALL was 0.19±0.06, which was significantly lower than that in control groups (P<0.05). There was significantly positive correlation of the expression level of PTEN gene with white blood cell count （r=0.993）and peripheral blood immature cells （r=0.996） in patients with T-ALL. There was no correlation between PTEN gene expression level and sex, age, LDH level, Hb level, platelet count in patients with T-ALL. And it was found that expression levels PTEN protein in the middle-risk group, the standard-risk group and the high-risk group were significant lower than those in the control group (P<0.05), while those in the high-risk group were significantly lower than those in other groups (P<0.05). Kaplan-Meier survival analysis showed that OS and DFS in PTEN gene high expression group were higher than those in PTEN low expression group (P<0.05). Cox multivariate regression analysis showed that white blood cell count and PTEN gene were independent influencing factors of OS (P<0.05); platelet count and PTEN gene were independent influencing factors of DFS (P<0.05).
CONCLUSION:PTEN gene level relates to the prognosis of patients with T-ALL. Patients with better prognosis show a higher PTEN gene level, which provides reference for clinical treatment of patients with T-ALL.
