Primary Mechanisms of CD34CD38--KG1a Leukemia Stem Cells Apoptosis Induced by FA-2-b-β Extracted from Qinba Selenium- Mushroo.
10.7534/j.issn.1009-2137.2019.06.009
- Author:
Dong-Ping WANG
1
;
Wei SHI
2
;
Wan-Wen GE
2
;
Ju-Xia ZHANG
1
;
Lian-Ping ZHAO
1
;
Xue CHEN
1
;
Li DONG
3
;
Yan-Qing SUN
4
Author Information
1. Department of Clinical Teaching, Gansu Provincial People's Hospital, Lanzhou 730000, Gansu Province, China.
2. Department of Urology, The Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China.
3. Department of Clinical Teaching, Gansu Provincial People's Hospital, Lanzhou 730000, Gansu Province, China,E-mail:donglijustgood@126.com.
4. Department of Clinical Teaching, Gansu Provincial People's Hospital, Lanzhou 730000, Gansu Province, China,E-mail:40yanqingfang@163.com.
- Publication Type:Journal Article
- MeSH:
ADP-ribosyl Cyclase 1;
Antigens, CD34;
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Membrane Glycoproteins;
Neoplastic Stem Cells;
Selenium
- From:
Journal of Experimental Hematology
2019;27(6):1761-1766
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the apoptosis of CD34CD38-KG1a leukemia stem cells induced by Qinba selenium-mushroom extract(FA-2-b-β), and its related mechanism.
METHODS:CD34CD38--KG1a cells were isolated from KG1a cell line by magnetic activated cell sorting. The proliferation ability of KG1a stem cells treatd by various concentration of FA-2-b-β(1.2-2.4 mg/ml) in vitro for 24 and 48 hours were tested by cell counting Kit-8(CCK8). Flow cytometry was used to detect the apoptosis rate of KG1a stem cells in each group after treated by FA-2-b-β in vitro. Expression of BAX,BCL-2,Casepase-3 and Cyclin D1 protein were detected by Western blot.
RESULTS:The proportion of CD34CD38--KG1a stem cells was (95.35±2.63）% after immunomagnetic isolation. The proliferation of KG1a stem cells was inhibited significantly by FA-2-b-β, which shows a time- and dose-dependent manner (24 h,r=0.943; 48 h,r=0.976). Flow cytometry shows that with the increasing of drug concentration, the apoptosis was also increased, when KG1a stem cells was treated by FA-2-b-β for 24 h. Western blot indicated that the expression of apoptosis-related protein BAX and Casepase-3 were up-regulated, the expression of BCL-2 and Cyclin D1 were down-regulated.
CONCLUSION:FA-2-b-β can regulate proliferation and apoptosis KG1a stem cells, the involved mechanism may be related with the activation of mitochondrial-mediated apoptotic pathway.
