Role of Reactive Oxygen Species in GDC-0152-Induced Apoptosis and Autophagy of NB4 cells.
10.7534/j.issn.1009-2137.2019.06.013
- Author:
Chuan LI
1
;
Wei JIANG
2
;
Zhuo-Gang LIU
1
;
Pei-Qi LIANG
1
;
Rong HU
3
Author Information
1. The First Department of Hematology, Shengjing Hospital, China Medical University，Shenyang 110031, Liaoning Province, China.
2. Department of Hematology, Benxi Central Hospital, Benxi 117000, Liaoning Province，China.
3. The First Department of Hematology, Shengjing Hospital, China Medical University，Shenyang 110031, Liaoning Province, China,E-mail：hur@sj-hospital.org.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Autophagy;
Cell Line, Tumor;
Cyclohexanes;
Humans;
Leukemia, Promyelocytic, Acute;
Pyrroles;
Reactive Oxygen Species
- From:
Journal of Experimental Hematology
2019;27(6):1786-1793
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of reactive oxygen species (ROS) on GDC-0152-induced apoptosis and autophagy of acute promyelocytic leukemia cell line NB4.
METHODS:Different concentrations of GDC-0152 combined with Z-VAD-FMK was applied to NB4 cells. Cell proliferation was detected by CCK8 method. Apoptosis rate, autophagy and ROS level were detected by flow cytometry. The autophagy was observed by Cyto-ID staining fluorescence microscopy, and flow cytometry were used to detect the fluorescence expression. The expression of autophagy-related protein LC3B was detected by Western blot.
RESULTS:GDC-0152 increased proliferation inhibition rate and apoptosis rate in NB4 cells (P＜0.05); GDC-0152 induced increase of ROS level of NB4 cells; GDC-0152 increased autophagy of NB4 cells that was found by Cyto-ID staining fluorescence microscopy and flow cytometry (P＜0.05). Western blot showed that GDC-0152 increased LC3B expression in NB4 cells and promoted the conversion of LC3BI to LC3BII; as compared with GDC-0152 (100 ng/ml), GDC-0152 (100 ng/ml) combined with ROS inhibitor YCG063 (10 μmol/L) decreased apoptosis and autophagy (P＜0.05).
CONCLUSION:GDC-0152 inhibits cell proliferation by inducing apoptosis and autophagy of NB4 cells. ROS can promote GDC-0152-induced apoptosis and autophagy of NB4 cells.
