Janus kinase 3 facilitates the migration of breast cancer cells by store-operated calcium channel.
- Author:
Xia LIU
1
;
Ting WEI
1
;
Zhao-Di GAO
1
;
Xiu-Liang ZHAO
1
;
Hui-Qing WU
1
;
Jing YAN
1
Author Information
1. Department of Physiology, Institute of Basic Medicine, Jining Medical University, Jining 272067, China.
- Publication Type:Journal Article
- MeSH:
Breast Neoplasms;
enzymology;
Calcium;
metabolism;
Calcium Channels;
metabolism;
Cell Movement;
physiology;
Gene Expression Regulation, Neoplastic;
Humans;
Janus Kinase 3;
genetics;
metabolism;
MCF-7 Cells;
ORAI1 Protein;
genetics
- From:
Acta Physiologica Sinica
2019;71(6):874-882
- CountryChina
- Language:Chinese
-
Abstract:
The present study was aimed to investigate the effect of Janus kinase 3 (JAK3) on the migration of breast cancer cells and the underlying mechanism. The expression of JAK3 in breast cancer MCF-7 cells was silenced by siRNA (siJAK3). The migration ability of MCF-7 cells was detected by scratch test. The activity of store-operated calcium channel (SOCC) was detected by fluorescence calcium imaging. The expression levels of Orai1 and STIM1, key molecules in the process of store-operated calcium entry (SOCE) were detected by Western blot and RT-PCR. The results showed that 2-APB, an inhibitor of SOCC, could inhibit the migration ability of MCF-7 cells. siJAK3 transfection significantly inhibited the migration ability of MCF-7 cells, decreased the activity of SOCC, and down-regulated mRNA and protein expression levels of Orai1 and Stim1. Over-expression of Orai1 or STIM1 in JAK3-silenced cells restored their migration ability. These results suggest that JAK3 facilitates the migration of breast cancer cells by SOCC.
