Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish.
10.1007/s11655-019-3204-6
- Author:
Junyoung OH
1
;
Dong Hyun KIM
2
;
Gi-Young KIM
1
;
Eun-Jin PARK
3
;
Jong Hoon RYU
4
;
Ji Wook JUNG
5
;
Se Jin PARK
6
;
Gwang-Woo KIM
7
;
Seungheon LEE
8
Author Information
1. Department of Marine Life Science, College of Ocean Science, Jeju National University, Jeju, 63243, Republic of Korea.
2. Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 604-714, Republic of Korea.
3. Institute of Convergence Bio-Health, Dong-A University, Busan, 604-714, Republic of Korea.
4. Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul, 130-701, Republic of Korea.
5. Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University, Yugok-dong, Gyeongsan, 712-715, Republic of Korea.
6. School of Natural Resources and Environmental Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea.
7. Marine Bio Convergence Center, Gangneung Science & Industry Promotion Agency, Gangneung, 25440, Republic of Korea.
8. Department of Marine Life Science, College of Ocean Science, Jeju National University, Jeju, 63243, Republic of Korea. slee76@jejunu.ac.kr.
- Publication Type:Journal Article
- Keywords:
Hydrangeae Dulcis Folium;
behavioural tests;
net handling stress;
whole-body cortisol;
zebrafish
- From:
Chinese journal of integrative medicine
2020;26(2):130-137
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To determine the effects of Hydrangeae Dulcis Folium (EHDF) on physical stress, changes in the whole-body cortisol level and behaviour in zebrafish (Danio rerio).
METHODS:One hundred and seventy-four fish were randomly divided into 4 [adrenocorticotropin hormone (ACTH) challenge test: 4 fish per group] or 6 groups (behavioural test: 10-12 fish per group, whole-body cortisol: 4 fish per group). Net handling stress (NHS) was used to induce physical stress. Fish were treated with vehicle or EHDF (5-20 mg/L) for 6 min before they were exposed to stress. And then, fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests, including novel tank test and open field test, and were evaluated to observe anxiety-like behaviours and locomotion. In addition, to elucidate the mode of action of the anti-stress effects of EHDF, ACTH (0.2 IU/g, i.p.) challenge test was performed.
RESULTS:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5-20 mg/L (P <0.05). Moreover, compared with the unstressed group, which was not treated with NHS, the whole-body cortisol level was significantly increased by treatment with NHS (P <0.05). Compared with the NHS-treated stressed control group, pre-treatment with EHDF at concentrations of 5-20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level (<0.05). In addition, ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion (P <0.05).
CONCLUSION:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.
