Factor V Leiden Gene Mutation in Femoral Head Osteonecrosis.
- Author:
Shin Yoon KIM
1
;
Jang Soo SUH
;
Eu Kyun PARK
;
Woon Bok JUNG
;
Jung Wan KIM
;
Kyung Hoi KOO
;
Chang Yoon KIM
Author Information
1. Department of Orthopedic Surgery, School of Medicicne, Kyungpook National University. syukim@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Femoral Head;
Osteonecrosis;
factor V Leiden
- MeSH:
Activated Protein C Resistance;
Digestion;
DNA;
Electrophoresis;
Exons;
Factor V*;
Head*;
Humans;
Leukocytes;
Osteonecrosis*;
Polymerase Chain Reaction;
Prevalence;
Sepharose;
Thrombophilia
- From:Journal of Korean Orthopaedic Research Society
2003;6(2):259-264
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of the present study was to evaluate the potential association of the G1691A mutation of factor V (factor V Leiden), which is a main causative factor of activated protein C resistance leading to intravascular coagulation, with osteonecrosis (ON) of the femoral head. MATERIALS AND METHODS: Genomic DNA was extracted from peripheral blood leukocytes of 116 consecutively identified patients with nontraumatic ON of the femoral head and 59 healthy controls. The region in exon 10, that encodes an APC cleavage site in factor V gene, was amplified by polymerase chain reaction (PCR) with use of the 2 primers (Korea Biotech Inc., Daejeon): 5'-GGA ACA ACA CCA TGA TCA GAG CA-3' (forward primer) and 5'-TAG CCA GGA GAC CTA ACA TGT TC-3'(reverse primer). Amplified product was subjected to MnlI restriction enzyme digestion and resulting fragments were separated by electrophoresis on 3% agarose gel. The homozygous and heterozygous patterns of DNA fragments of 1691G-A mutation in the factor V gene was investigated. RESULTS: The prevalence of factor V Leiden was 0% in the patients group and in the control group. CONCLUSIONS: The data suggested that thrombophilia by the G1691A mutation of factor V (factor V Leiden) was less likely to be associated with the development of ON of the femoral head in Koreans.
