Expression of miR-155-5p in Wilms tumor and its regulatory role in proliferation, migration and apoptosis of Wilms tumor cells .
10.12122/j.issn.1673-4254.2019.12.12
- Author:
Xin LUO
1
,
2
;
Junjun DONG
1
,
2
;
Xingyue HE
1
,
2
;
Lianju SHEN
1
,
2
;
Chunlan LONG
1
,
2
;
Feng LIU
1
,
2
;
Xing LIU
1
,
2
;
Tao LIN
1
,
2
;
Dawei HE
1
,
2
;
Guanghui WEI
1
,
2
Author Information
1. Department of Pediatric Urologic Surgery, Children's Hospital of Chongqing Medical University
2. Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China.
- Publication Type:Journal Article
- Keywords:
Wilms tumor;
apoptosis;
miR-155-5p;
migration;
proliferation
- MeSH:
Apoptosis;
Cell Movement;
Cell Proliferation;
Humans;
Kidney Neoplasms;
genetics;
MicroRNAs;
genetics;
Neoplasm Invasiveness;
Wilms Tumor;
genetics
- From:
Journal of Southern Medical University
2019;39(12):1476-1481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:explore the expression of miR-155-5p in Wilms tumor and its effect in regulating the proliferation, migration and apoptosis of Wilms tumor cells.
METHODS:Specimens of tumor tissues and paired adjacent tissues were obtained from 40 patients with Wilms tumor for detection of the expression levels of miR-155-5p using RT-qPCR. Wilms tumor cell line G401 was transfected with miR-155-5p mimics and miR-155-5p inhibitor to induce miR-155-5p over-expression and its inhibition, respectively, and the changes in the cell proliferation, migration and apoptosis were assessed using cell counting kit-8 (CCK-8), wound healing assay and fl ow cytometry.
RESULTS:RT-qPCR showed that the expression of miR-155-5p decreased significantly in Wilms tumor tissues as compared with normal kidney tissues and was significantly associated with TNM stage ( < 0.05). In G401 cells, over-expression of miR-155-5p significantly inhibited the cell proliferation and migration and promoted cell apoptosis ( < 0.05), and down-regulation of miR-155-5p obviously enhanced the proliferation and migration and suppressed apoptosis of the cells ( < 0.05).
CONCLUSIONS:miR-155-5p is down-regulated in Wilms tumor and its expression level is correlated with TNM stage. miR-155-5p participates in the progression of Wilms tumor by inhibiting the proliferation and migration and promoting apoptosis of the tumor cells, and may serve as a novel biomarker for diagnosis, therapy and prognostic evaluation of Wilms tumor.
