The regulatory mechanisms of IGF1 in the osteogenic differentiation of canine MSMSCs via BMP2⁃Smad 1/5 signaling pathway
10.12016/j.issn.2096⁃1456.2020.01.003
- Author:
LIAO Chunhui
1
,
2
;
LI I Mingfei
3
;
YE Jinmei
1
,
2
;
PENG Wei
3
;
CHEN Songling
3
Author Information
1. Department of Or⁃thodontics, Guangzhou Women and Children&prime
2. s Medical Center
3. Department of Stomatol⁃ogy, The First Affiliated Hospital, SunYat⁃sen University
- Publication Type:Journal Article
- Keywords:
insulin⁃like growth factor 1;
maxillary sinus membrane stem cells;
BMP2⁃Smad1/5 signaling path⁃
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(1):16-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of the bone morphogenetic protein 2 (BMP2)⁃Smad1/5 and p38MAPK
signaling pathways in the osteogenic differentiation of MSMSCs by insulin⁃like growth factor 1 (IGF1).
Methods :A re⁃
combinant adenovirus (RAD) and IGF1 expressing IGF1 gene were constructed. After osteogenic induction, qRT⁃PCR
and Western blot were used to detect the phosphorylation level of Smad1/5 and the expression of the BMP⁃2 protein in
the BMP⁃Smad signaling pathway; immunohistochemistry was used to observe the nuclear translocation of Smad1/5; qRT⁃PCR and Western blot were used to detect IGF with Noggin and SB203580, inhibitors of the p38MAPK signaling path⁃
way 1⁃mediated osteogenic differentiation of MSMSCs
Results:The recombinant IGF1 adenovirus was constructed suc⁃
cessfully. MSMSCs were cultured in inductive medium after infection with different concentrations of Ad⁃IGF1, and
then, the protein levels of BMP2 and p⁃Smad1/5 increased. IGF1 can also induce nuclear translocation of Smad1/5. In
addition, Noggin significantly reduced the phosphorylation level of Smad1/5 and the formation of mineralized nodules in
the MSMSCs. The mRNA levels of Runx2, OPN and ALP also decreased. In contrast, SB203580 decreased neither the
phosphorylation level of p38 nor the mRNA expression of Runx2, OPN and ALP in the Ad⁃IGF1 MSMSCs
Conclu⁃sion:IGF1 can promote the osteogenic differentiation of MSMSCs via the BMP2⁃Smad1/5 signaling pathway. In con⁃
trast, IGF1 may not promote the osteogenic differentiation of MSMSCs via the p38MAPK signaling pathway.
- Full text:IGF1通过BMP2-Smad1_5信号通路调控犬上颌窦黏膜干细胞成骨分化.pdf
