The study of atmospheric particulate matters and IFN-γDNA methylation in CD4⁺ T cells from patients with AR children
10.13201/j.issn.1001-1781.2016.07.004
- Author:
You Jin LI
1
;
Niu LI
2
;
Zhe MU
3
;
Beiying MA
2
;
Fan JIANG
4
;
Jie CHEN
1
Author Information
1. Department of Otorhinolaryngology, Shanghai Children's Medical Center Affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China
2. Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine
3. Shanghai Meteorological Bureau
4. Department of Developmental and Behavioral Pediatrics, Shanghai Children's Medical Center Affiliated with Shanghai Jiaotong University School of Medicine, MOE-Shanghai Key Laboratory of Children's Environmental Health
- Publication Type:Journal Article
- Keywords:
children;
rhinitis allergic;
particulate matter;
lymphoma;
IFN-γgene methlyation;
polarity of Th2
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2016;30(7):523-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the possible effects of meteorological and environmental factors on AR of children and IFN-γgene specific DNA methylation levels in CD4⁺ T cells of patients with AR. Method:Undergoing follow-up on 35 pediatric AR patients (6-12 years). Data on daily sulfur dioxide (SO₂), nitrogen dioxide (NO₂), particulate matter of diameter smaller than 10 micrometer (PM-10) and particulate matter of diameter smaller than 2.5 micrometer (PM2.5), the average of ozone (O₃) per 8 hours was available as average values derived from the data of 6 state controlled monitoring stations distributed across Pudong district, Shanghai. We quantified IFN-γ (interferon-γ) gene specific DNA methylation levels in CD4⁺ T cells from 35 patients with AR and 30 healthy controls. mRNA levels of IFN-γ gene were measured by real-time reverse transcriptase-PCR. Methods of personal exposure assessment of PM2.5 and PM10 were measured. Result:Compared with control, IFN-γ promoter region was hypermethylated in AR CD4⁺ T cells (P<0.05). Of all observed CpG sites in IFN-γ promoter region, there were significant differences in CpG⁻²⁹⁹, CpG⁺¹¹⁹, CpG⁺¹⁶⁸ (P=0.004, P=0.029, P=0.035). IFN-γ mRNA expression was significantly increase in CD4⁺ T cells (P<0.05). The level of IFN-γ mRNA expression was negatively correlated to mean level of methylation in IFN-γ promoter region. After adjusting, level of long exposure PM2.5 was positively correlated with level of methylation in IFN-γ promoter region. Conclusion:Level of methylation in IFN-γ promoter region may be affected by long exposure PM2.5.
