Identification of pathogenic bacteria on the salted fish Lutjanus vivanus in Sorong City of West Papua
- Author:
Sukmawati
1
;
Fatimah Hardianti
1
;
Sipriyadi
2
;
Isna Rasdianah Aziz
3
Author Information
- Publication Type:Journal Article
- Keywords: Lutjanus vivanus; pathogenic bacteria; West Papua
- From:Malaysian Journal of Microbiology 2019;15(3):237-244
- CountryMalaysia
- Language:English
-
Abstract:
Aims:Fisheries-based food is needed by humans as a protein source, one of which is salted fish Lutjanus vivanus. Market demand for this product in Indonesia is quite high. The aim of the study was to identify pathogenic bacteria in salted fish L. vivanus.
Methodology and results:The method used in this study was descriptive method including pathogenic test, bacterial genomic DNA isolation based on 16 sRNA gene using prokaryotic specific primers, namely 63f forward primer (5′-CAG GCC TAA CAC ATG CAA GTC-3′) and reverse 1387r primer (5′-GGG CGG WGT GTA CAA GGC-3′). The results of this study indicated pathogenic bacteria in salted fish L. vivanus with pathogenic activity of α hemolytic and β hemolytic. The bacteria were identified as Serratia marcescens strain ZK2 16S for isolate KS and Bacillus altitudinis strains A-19 16S for isolate LG.
Conclusion, significance and impact of study:In salted fish L. vivanus in the Sorong city, West Papua, it was obtained Serratia marcescens strain ZK2 16S with total hemolytic activity and B. altitudinis A-19 16S strain with partial hemolytic activity. - Full text:20.2019my0536.pdf
