In vitro Antibacterial Properties of Etlingera elatior Flower Extracts against Acne-Inducing Bacteria: Propionibacterium acnes and Staphylococcus aureus
- Author:
Nurul Saidah D
- Collective Name:Salwani I, Mohd Hilmi AB, Mohd Khairi Z , Rabiatul Adawiyah U , Nurul ‘Adani S
- Publication Type:Journal Article
- Keywords:
Antibacterial properties, Etlingera elatior flower, Acne-inducing bacteria.
- From:
The International Medical Journal Malaysia
2019;18(3):128-135
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Acne is a common skin disorder and is generally caused by Propionibacterium acnes and
Staphylococcus aureus. Etlingera elatior flower extract is known to have antibacterial properties however,
the properties against these bacteria have not been extensively reported. Thus, this study aimed to
investigate the antibacterial properties of the flower extract against these bacteria. Materials and
Methods: The flower extract was subjected to sequential extraction using three different solvent
polarities; n-Hexane, dichloromethane (DCM) and ethanol. The antibacterial properties were evaluated
using the disc diffusion and broth dilution assays techniques by determining the inhibition zone diameter,
minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Total phenolic
acids (TPC) and flavonoids contents (TFC) were estimated using Folin-Ciocalteu and aluminium-chloride
colorimetric assay respectively. Morphological changes of the treated bacteria were studied using scanning
electron microscope (SEM). Results: DCM flower extract showed the highest antibacterial properties against
P. acnes; at 25 mg/ml it had the widest inhibition zone (11.39 ± 0.45 mm) and the lowest MIC (6.25 mg/
mL) and MBC (12.5 mg/mL). The ethanolic flower extract had the highest antibacterial properties against
S. aureus; at 50 mg/ml the inhibition zone was 6.21 ± 0.25 mm and the MIC and MBC were both 12.5 mg/
mL. Ethanolic extracts had the highest TPC (966.304 ± 114.08 mg GAE/g) and TFC (796.33 ± 65.78 mg QE/
g). There was significant morphological changes of the treated bacteria observed under SEM. Conclusion: E.
elatior flower extracts exhibited antibacterial properties against acne-inducing bacteria.
- Full text:180316.pdf
