Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/ Reperfusion Injury

Bahar Mazaheri; Fatemeh Emami; Fatemeh Moslemi; Ardeshir Talebi; Mehdi Nematbakhsh

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Zinc Supplementation and Ischemia Pre-conditioning in Renal Ischemia/ Reperfusion Injury

Author: Bahar Mazaheri ¹ ; Fatemeh Emami ¹ ; Fatemeh Moslemi ¹ ; Ardeshir Talebi ² ; Mehdi Nematbakhsh ³
Author Information

1. Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
3. IsfahanMN Institute of Basic and Applied Sciences Research, Isfahan, Iran
Publication Type:Journal Article
Keywords: Keywords; renal ischemia/reperfusion, zinc, pre-conditioning ischemia, nephrectomy, rat
From:Malaysian Journal of Medical Sciences 2019;26(4):39-46
CountryMalaysia
Language:English
Abstract: Backgrounds: Renal ischemia/reperfusion (RIR) is a major cause of kidney dysfunction in clinic. The main objective of this study was to investigate the effect of pre-conditioning ischemia (IPC) and zinc (Zn) supplementation on renal RIR injury. Methods: A total of 63 unilateral nephrectomised male and female Wistar rats were divided into five groups. Group 1 (ShOPR): Rats as sham-operated group were subjected to surgical procedure without RIR. Group 2 (Isch): Rats underwent RIR (left kidney ischemia for 30 min followed by 48 h reperfusion). Group 3 (Zn+Isch): Rats were treated as group 2 but they received Zn sulphate (30 mg/kg) 1 h before induction of RIR. Group 4 (IPC+Isch): Rats were treated as group 2 but they underwent 1 min of ischemia followed by 3 min reperfusion as IPC, which was repeated for three times before induction of RIR. Group 5 (Zn+IPC+Isch): Rats were subjected to receive both Zn sulphate and IPC before induction of RIR. Urine samples were collected in the last 6 h of reperfusion, and finally biochemical and histological measurements were performed. Results: The serum level of creatinine (Cr), normalised kidney weight (KW) and kidney tissue damage score (KTDS) increased by RIR alone significantly (P < 0.05). These parameters were attenuated statistically by Zn supplementation (P < 0.05). However, IPC alone or cotreatment of Zn and IPC did not improve the biochemical and histological markers altered by RIR injury. Conclusion: Zn supplementation had a protective role against RIR while such protective effect was not observed by IPC alone or by co-treatment of Zn and IPC.
Full text:3.2019my0798.pdf