In-vitro Study: Camp Overshoot Caused by Chronic Morphine Alleviated by the Synergistic Combination of Zamzam Water and Methadone in Human Primary Glioblastoma Cell Line (U-87 MG)
- Author:
Shariff Halim
- Collective Name:Nasir Mohamad, Ridzuan PM , Nur Husna Zakaria , Nur Atikah Muhammad
- Publication Type:Journal Article
- Keywords:
Morphine, cAMP, Zamzam Water, Methadone
- From:
The International Medical Journal Malaysia
2019;18(2):47-52
- CountryMalaysia
- Language:English
-
Abstract:
Introduction: Zamzam water is blessed water originated from Mecca and is believed by Muslims to have
the ability to cure illness. This ability to cure illness is due to the fact that zamzam water has higher
concentration of minerals especially sodium, calcium and magnesium which play a vital role. Sodium is
reported to be involved in the regulation of the Mu-Opioid Receptor, which indirectly leads to the production
of cAMP. Hence, this current study was carried out to investigate the synergistic effects of zamzam water
and methadone combination on cAMP levels in human primary glioblastoma cell line (U-87 MG) after chronic
morphine administration. Materials and Methods: The time course and concentration of morphine on U-87 MG
cell line was determined. The U-87 MG cell line was incubated with morphine (25 µL/mL) for 24h, to make
the cell dependent on morphine and later treated with different combinations of 3.2 mL of zamzam water
and methadone (5, 10, 25 µL/mL). The levels of cAMP was determined using the enzyme-linked
immunosorbent assay kit. Results: The result revealed that 3.2 mL of zamzam water incubated with 10 µl/
mL of methadone significantly prevented the overshoot production of cAMP level (p<0.05) in U-87 MG cell
line after 48h incubation when compared to the untreated samples. Conclusion: These finding suggest that
co-treatment with zamzam water and methadone could possibly avoid tolerance and dependence on chronic
morphine treatment by preventing the up-regulation of cAMP level.
- Full text:180206.pdf
